Injectable hyaluronic acid hydrogels via Michael addition as dermal fillers for skin regeneration applications

Area of Science:

• Biomaterials Science
• Dermatology
• Tissue Engineering

Background:

• Hyaluronic acid (HA) is a key component in skin structure and hydration.
• Dermal fillers aim to restore lost volume and improve skin texture.
• Existing fillers face challenges in biocompatibility, longevity, and tissue integration.

Purpose of the Study:

• To develop novel injectable hyaluronic acid-based hydrogels for dermal filler applications.
• To optimize hydrogel properties through controlled crosslinking and formulation.
• To evaluate the biocompatibility and tissue remodeling potential of these hydrogels in a simulated dermal environment.

Main Methods:

• Synthesis of thiolated hyaluronic acid and its crosslinking with polyethylene glycol derivatives via Michael-type addition.
• Formulation of biphasic gels with crosslinked HA microparticles in a fluid matrix.
• Characterization of hydrogel properties: enzymatic degradation, injectability, and rheology.
• In vitro assessment using a 3D culture model to simulate dermal remodeling and inflammatory response.

Main Results:

• Optimized thiol substitution allowed tuning of crosslinking kinetics and hydrogel properties.
• Biphasic gel formulation improved injectability and handling characteristics.
• Hydrogels demonstrated balanced collagen synthesis and degradation in the 3D model, indicating potential for skin regeneration.
• Controlled inflammatory response and reduced adverse effects were observed, supporting tissue repair.

Conclusions:

• The developed hyaluronic acid hydrogels are promising candidates for safe and effective dermal fillers.
• These materials support tissue remodeling and manage inflammation, crucial for aesthetic and reconstructive applications.
• Further in vivo studies are warranted to confirm efficacy and long-term safety in dermal filler applications.