LM-GlycoRepo Version 1.0: A Novel Repository System for Mouse Tissue Glycome Mapping Data

Area of Science:

• Glycomics
• Biotechnology
• Data Science

Background:

• Lectin microarray (LMA) is a sensitive method for profiling protein glycosylation.
• Growing use of LMA necessitates a data repository adhering to FAIR principles (Findable, Accessible, Interoperable, Reusable).

Purpose of the Study:

• To introduce LM-GlycoRepo, a novel repository for lectin-based multimodal (LM) data.
• To establish a system compliant with the MIRAGE (Minimum Information Required for a Glycomics Experiment) guideline.

Main Methods:

• LM-GlycoRepo Version 1.0 is specialized for mouse tissue glycome mapping data.
• Data includes laser microdissection (LMD) images, LMA data, and lectin staining images.
• An embargo system is incorporated for data release control.

Main Results:

• LM-GlycoRepo enables deposition of multimodal LM data.
• Deposited data can be visualized via LM-GlycomeAtlas post-embargo.
• The system supports standardized LMD-assisted LMA procedures.

Conclusions:

• LM-GlycoRepo provides a FAIR-compliant repository for LMA data.
• The repository facilitates data sharing and reuse in glycomics research.
• Future versions will accommodate diverse biospecimens, including human samples.